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Project 2

Yeast Genetics and Stress Response Genes

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Dr. Paul Russell

To understand how eukaryotic organisms respond to hazardous chemicals found at Superfund sites, project investigators are using genetic and biochemical studies to develop a sensitive yeast growth assay that signals the presence of environmental toxins. These studies will utilize the fission yeast, Schizosaccharomyces pombe. Fission yeast has served as an outstanding model system in previous studies of cellular responses to genotoxic and cytotoxic agents. Recent studies of fission yeast have uncovered the stress-activated protein kinase (SAPK) Spc1 and transcription factors Pap1 and Atf1 that resemble stress response factors in higher eukaryotes. These proteins regulate the transcriptional induction of stress response genes. DNA microarray technologies, combined with the genome sequence of S. pombe, will provide the opportunity to develop a comprehensive picture of the transcriptional response to stress in fission yeast.

Main Contact info

Dr. Paul Russell, Project Leader
The Scripps Research Institute
Department of Molecular Biology, MB3
10550 North Torrey Pines Road
La Jolla, CA 92037
P: 858-784-8273
F: 858-784-2265
E-mail:

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Recent Publications and Documents

Mendoza-Cozatl, D., Zhai, Z., Jobe, T., G.Z., A., Song, W. Y., Limbo, O., Russell, M., Kozlovskyy, V., Martinoia, E., Vatamaniuk, O. K., Russell, P. & Schroeder, J. I. 2010. Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance. J. Biol. Chem. 285, 40416-40426. PMC3003340
PubMed ID: 20937798

Kennedy PJ, Vashisht AA, Hoe KL, Kim DU, Park HO, Hayles J, Russell P. A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.Toxicol Sci. 106(1):124-39, 2008. PMC2563147
PubMed ID: 18684775

Rodriguez-Gabriel, MA, Russell, P. Control of mRNA stability by SAPKs. Topics Curr. Genet. 20: 50-166, 2008.
PubMed ID: Link to Pub

Vashisht AA, Kennedy PJ, Russell P. Centaurin-like protein Cnt5 contributes to arsenic and cadmium resistance in fission yeast. FEMS Yeast Res. 9, 257-269, 2009. PMC2820371
PubMed ID: 19076239

Martín V, Rodríguez-Gabriel MA, McDonald WH, Watt S, Yates 3rd JR, Bähler J, Russell P. Cip1 and Cip2 are novel RNA-Recognition-Motif proteins that counteract Csx1 function during oxidative stress. Mol. Biol. Cell 17:1176-1183, 2006.
PubMed ID: 16407405

Rodríguez-Gabriel MA, Watt S, Bähler J, Russell P. Upf1, an RNA helicase required for nonsense-mediated mRNA decay, modulates the transcriptional response to oxidative stress in fission yeast. Mol. Cell. Biol. 26: 6347-6356, 2006.
PubMed ID: 16914721

Rodriguez-Gabriel MA, Russell P. Distinct signaling pathways respond to arsenite and reactive oxygen species in Schizosaccharomyces pombe. Eukaryotic Cell 4(8):  1396-1402, 2005.
PubMed ID: 16087744