Molecular Mechanisms of Heavy Metal Detoxification and Engineering Accumulation in Plants

Project 6

Molecular Mechanisms of Heavy Metal Detoxification and Engineering Accumulation in Plants

Project Summary/Abstract
Soils and waters with high levels of toxic metal(loid)s such as cadmium, lead, arsenic and mercury are detrimental to human and environmental health. These 4 heavy metal(loid)s are among the Superfund's top 7 priority hazardous substances. Many human diseases have been attributed to environmental contamination by heavy metals, including cancers and neurological disorders. Research and applications indicate that uptake of heavy metals into plant roots and accumulation of heavy metals could provide a cost effective approach for toxic metal removal and bioremediation of heavy metal-laden soils and waters. In recent research we have made major advances at understanding key mechanisms that function in heavy metal detoxification, transport and accumulation in plants. However important genes and pathways that function in heavy metal over-accumulation in plants remain to be identified. We will combine powerful genomic, genetic, biochemical and engineering approaches to test new central hypotheses by pursuing the following Specific Aims:
Aim I. The regulatory mechanisms, transcription factors (TFs), and transcriptional network that mediate rapid heavy metal(loid)-induced transcriptional responses in plants remain largely unknown. Using a luciferase-based cadmium- and arsenic-induced reporter mutant screening approach we have isolated mutants in rapid Cd- and As-induced gene expression. New mutants in major Cd-/As-dependent repression and induction loci will be characterized and the underlying genes isolated and their functions determined. Collaborative research with Geoffrey Chang (Project 5) will pursue development of cost-effective innovative heavy metal toxicant nano-reporters in plants.
Aim II. The many genetic redundancies in plant genomes cause major limitations in heavy metal response gene discovery. To address redundant gene function on a systems biology scale we have designed a genomic scale artificial microRNA (amiRNA) library for genome-wide knockdown of homologous gene family members which is leading to discovery of new genes and will be used to characterize key plant genes and network mechanisms that function in heavy metal accumulation, resistance and remediation.
Aim III: Using genes identified in Specific Aims I and II and previous research, gene-stacking will be used to generate plants and investigate their enhanced heavy metal accumulation and root sequestration (phytostabilization) potential. Furthermore, by genomic investigation of plants that are being used for phytostabilization at semi-arid Superfund sites, the above advances will be used in collaboration with the University of Arizona Superfund Research Center to uncover mechanisms that render plants suitable for phytostabilization of toxic metal(loid)s. The proposed research will be leveraged to develop technologies for avoiding the growing problem of accumulation of heavy metals and arsenic in edible plant tissues.

Publication

PubMed Central ID: 

Azoulay-Shemer T, Hsu PK, Schroeder JI. (2017) Seeing is believing. Nat Plants. 3(10):765-766. doi: 10.1038/s41477-017-0025-5.

PubMed Central ID: 

Stephan A.B., Kunz H.H., Yang E., Schroeder J.I. (2016) Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters. Proc Natl Acad Sci U S A. 113(35):E5242-9. doi: 10.1073/pnas.1519555113. Epub 2016 Aug 15.

PubMed Central ID: 

Aleman F., Yazaki J., Lee M., Takahashi Y., Kim A.Y., Li Z., Kinoshita T., Ecker J.R., Schroeder J.I. (2016) An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling. Sci Rep. 6:28941. doi: 10.1038/srep28941.

PubMed Central ID: 

Yazaki J., Galli M., Kim A.Y., Nito K., Aleman F., Chang K.N., Carvunis A.R., Quan R., Nguyen H., Song L., Alvarez J.M., Huang S.S., Chen H., Ramachandran N., Altmann S., Gutiérrez R.A., Hill D.E., Schroeder J.I., Chory J., LaBaer J., Vidal M., Braun P., Ecker J.R. (2016) Mapping transcription factor interactome networks using HaloTag protein arrays. Proc Natl Acad Sci U S A. 113:E4238-47.
doi: 10.1073/pnas.1603229113.

PubMed Central ID: 

Suzuki, K., Yamaji, N., Costa, A., Okuma, E., Kobayashi, N. I., Kashiwagi, T., Katsuhara, M., Wang, C., Tanoi, K., Murata, Y., Schroeder, J. I., Ma, J. F., Horie, T. (2016) OsHKT1;4-mediated Na(+) transport in stems contributes to Na(+) exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. BMC Plant Biol. 16(1), 22. doi: 10.1186/s12870-016-0709-4.

PubMed Central ID: 

Yamamoto Y., Negi J., Wang C., Isogai Y., Schroeder J.I., Iba K. (2016) The Transmembrane Region of Guard Cell SLAC1 Channels Perceives CO2 Signals via an ABA-Independent Pathway in Arabidopsis. Plant Cell. 28:557-67. doi: 10.1105/tpc.15.00583.

PubMed Central ID: 

Wang, C., Hu, H., Qin, X., Zeise, B., Xu, D., Rappel, W.-J., Boron, W.F., Schroeder, J.I. (2016) Reconstitution of CO2 regulation of SLAC1 anion channel and function of CO2-permeable PIP2;1 aquaporin as CARBONIC ANHYDRASE 4 interactor. Plant Cell. 282: 568-82. doi: 10.1105/tpc.15.00637.

PubMed Central ID: 

Munemasa, S., Hauser, F., Park, J., Waadt, R., Brandt, B., Schroeder, J. I. (2015) Mechanisms of abscisic acid-mediated control of stomatal aperture. Curr Opin Plant Biol. 154-162. doi: 10.1016/j.pbi.2015.10.010.

PubMed Central ID: 
Waadt, R., Hsu, P. K., Schroeder, J. I. (2015) Abscisic acid and other plant hormones: Methods to visualize distribution and signaling. Bioessays. 37(12),1338-49. doi: 10.1002/bies.201500115.
PubMed Central ID: 

Engineer, C. B., Hashimoto-Sugimoto, M., Negi, J., Israelsson-Nordström, M., Azoulay-Shemer, T., Rappel, W. J., Iba, K., Schroeder, J. I. (2015) CO2 Sensing and CO2 Regulation of Stomatal Conductance: Advances and Open Questions. Trends Plant Sci. 21(1):16-30. doi: 10.1016/j.tplants.2015.08.014.

PubMed Central ID: 

Zhang, Z., Xie, Q., Jobe, T. O., Kau, A. R., Wang, C., Li, Y., Qiu, B., Wang, Q., Mendoza-Cózatl, D. G., Schroeder, J. I. (2015) Identification of AtOPT4 as a Plant Glutathione Transporter. Mol Plant. 9(3):481-484. doi: 10.1016/j.molp.2015.07.013.

PubMed Central ID: 

Brandt, B., Munemasa, S., Wang, C., Nguyen, D., Yong, T., Yang, P. G., Poretsky, E., Belknap, T. F., Waadt, R., Alemán, F., Schroeder, J. I. (2015) Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells. Elife. 4. doi: 10.7554/eLife.03599.

PubMed Central ID: 

Azoulay-Shemer, T., Palomares, A., Bagheri, A., Israelsson-Nordstrom, M., Engineer, C. B., Bargmann, B. O., Stephan, A. B., Schroeder, J. I. (2015) Guard cell photosynthesis is critical for stomatal turgor production, yet does not directly mediate CO2 - and ABA-induced stomatal closing. Plant J. 83(4), 567-81. doi: 10.1111/tpj.12916.

PubMed Central ID: 

Keinath, N. F., Waadt, R., Brugman, R., Schroeder, J. I., Grossmann, G., Schumacher, K., Krebs, M. (2015) Live Cell Imaging with R-GECO1 Sheds Light on flg22- and Chitin-Induced Transient [Ca(2+)]cyt Patterns in Arabidopsis. Mol Plant. 8(8),1188-200. doi: 10.1016/j.molp.2015.05.006.

PubMed Central ID: 

Engineer, C. B., Ghassemian, M., Anderson, J. C., Peck, S. C., Hu, H., Schroeder, J. I. (2014) Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development. Nature. 513(7517), 246-50. doi: 10.1038/nature13452.

PubMed Central ID: 

Mendoza-Cózatl, D.G., Xie, Q., Akmakjian, G. Z., Jobe, T. O., Patel, A., Stacey, M. G., Song, L., Demoin, D. W., Jurisson, S. S., Stacey, G., Schroeder, J. I. (2014) OPT3 is a component of the iron-signaling network between leaves and roots and misregulation of OPT3 leads to an over-accumulation of cadmium in seeds. Mol Plant. 7(9), 1455-69. doi: 10.1093/mp/ssu067.

PubMed Central ID: 

Kunz, H. H., Gierth, M., Herdean, A., Satoh-Cruz, M., Kramer, D. M., Spetea, C., Schroeder, J. I. (2014) Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis. Proc Natl Acad Sci USA. 111(20), 7480-5. doi: 10.1073/pnas.1323899111.

PubMed Central ID: 
Stephan, A. B., Schroeder, J. I. (2014) Plant salt stress status is transmitted systemically via propagating calcium waves.
Proc Natl Acad Sci U S A. 111(17), 6126-7. doi: 10.1073/pnas.1404895111.
PubMed Central ID: 
Waadt, R., Hitomi, K., Nishimura, N., Hitomi, C., Adams, S. R., Getzoff, E. D., Schroeder, J. I. (2014) FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis.
Elife. 3, e01739. doi: 10.7554/eLife.01739.
PubMed Central ID: 

Deinlein, U., Stephan, A. B., Horie, T., Luo, W., Xu, G., Schroeder, J. I. (2014) Plant salt-tolerance mechanisms. Trends Plant Sci. 19(6), 371-379. doi: 10.1016/j.tplants.2014.02.001.

PubMed Central ID: 

Song, W. Y., Mendoza-Cózatl, D. G., Lee, Y., Schroeder, J. I., Ahn, S. N., Lee, H. S., Wicker, T., Martinoia, E. (2014) Phytochelatin-metal(loid) transport into vacuoles shows different substrate preferences in barley and Arabidopsis. Plant Cell Environ. 37(5), 1192-201. doi: 10.1111/pce.12227.

PubMed Central ID: 

Hauser, F., Chen, W., Deinlein, U., Chang, K., Ossowski, S., Fitz, J., Hannon, G. J., Schroeder, J. I. (2013) A genomic-scale artificial microRNA library as a tool to investigate the functionally redundant gene space in Arabidopsis. Plant Cell. 25(8), 2848-63. doi: 10.1105/tpc.113.112805.

PubMed Central ID: 

McDowell, S. C., Akmakjian, G., Sladek, C., Mendoza-Cozatl, D., Morrissey, J. B., Saini, N., Mittler, R., Baxter, I., Salt, D. E., Ward, J. M., Schroeder, J. I., Guerinot, M. L., Harper, J. F. (2013) Elemental concentrations in the seed of mutants and natural variants of Arabidopsis thaliana grown under varying soil conditions. PLoS One. doi: 10.1371/journal.pone.0063014.

PubMed Central ID: 

Schroeder, J. I., Delhaize, E., Frommer, W. B., Guerinot, M. L., Harrison, M. J., Herrera-Estrella, L., Horie, T., Kochian, L. V., Munns, R., Nishizawa, N. K., Tsay, Y. F., Sanders, D. (2013) Using membrane transporters to improve crops for sustainable food production. Nature. 497(7447), 60-6. doi: 10.1038/nature11909.

PubMed Central ID: 

Jobe, T. O., Sung, D. Y., Akmakjian, G., Pham, A., Komives, E. A., Mendoza-Cózatl, D. G., Schroeder, J. I. (2012) Feedback inhibition by thiols outranks glutathione depletion: a luciferase-based screen reveals glutathione-deficient γ-ECS and glutathione synthetase mutants impaired in cadmium-induced sulfate assimilation. Plant J. 70(5), 783-95. doi: 10.1111/j.1365-313X.2012.04924.x.

PubMed Central ID: 

Xue, S., Yao, X., Luo, W., Jha, D., Tester, M., Horie, T., Schroeder, J. I. (2011) AtHKT1;1 mediates nernstian sodium channel transport properties in Arabidopsis root stelar cells. PLoS One. 6(9):e24725. doi: 10.1371/journal.pone.0024725.

PubMed Central ID: 

Mendoza-Cózatl, D.G., Jobe, T. O., Hauser, F., Schroeder, J. I. (2011) Long-distance transport, vacuolar sequestration, tolerance, and transcriptional responses induced by cadmium and arsenic. Curr Opin Plant Biol. 14(5), 554-62. doi: 10.1016/j.pbi.2011.07.004.

PubMed Central ID: 

Jannat, R., Uraji, M., Morofuji, M., Islam, M. M., Bloom, R. E., Nakamura, Y., McClung, C. R., Schroeder, J. I., Mori, I. C., Murata, Y. (2011) Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells. J Plant Physiol. 168(16),1919-26. doi: 10.1016/j.jplph.2011.05.006.

PubMed Central ID: 

Horie, T., Brodsky, D. E., Costa, A., Kaneko, T., Lo Schiavo, F., Katsuhara, M., Schroeder, J. I. (2011) K+ transport by the OsHKT2;4 transporter from rice with atypical Na+ transport properties and competition in permeation of K+ over Mg2+ and Ca2+ ions. Plant Physiol. 156(3), 1493-507. doi: 10.1104/pp.110.168047.

PubMed Central ID: 

​Hauser, F. Waadt, R. & Schroeder, J. I. (2011) Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol. doi: 10.1016/j.cub.2011.03.015.

PubMed Central ID: 

Böhmer, M. & Schroeder, J. I. (2011) Quantitative transcriptomic analysis of ABA-induced and ROSdependent expression changes and proteomic profiling in Arabidopsis suspension cells. Plant Journal. 67(1):105-18. doi: 10.1111/j.1365-313X.2011.04579.x. 

PubMed Central ID: 

Song, W. Y., Park, J., Mendoza-Cózatl, D.G., Suter-Grotemeyer, M., Shim, D., Hörtensteiner, S., Geisler, M., Weder, B., Rea, P. A., Rentsch, D., Schroeder, J. I., Lee, Y., Martinoia, E. (2010) Arsenic tolerance in Arabidopsis is mediated by two ABCC-type phytochelatin transporters. Proc Natl Acad Sci USA. 107, 21187-92. doi: 10.1073/pnas.1013964107.

PubMed Central ID: 

Mendoza-Cózatl, D. G., Zhai, Z, Jobe, T. O., Akmakjian, G. Z., Song, W. Y., Limbo, O., Russell, M. R., Kozlovskyy, V. I., Martinoia, E., Vatamaniuk, O. K., Russell, P., Schroeder, J. I. (2010) Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance. J Biol Chem. 285(52), 40416-26. doi: 10.1074/jbc.M110.155408.

PubMed Central ID: 

Costa, A., Drago, I., Behera, S., Zottini, M., Pizzo, P., Schroeder, J. I., Pozzan, T., Schiavao, F. L. (2010) H(2)O(2) in plant peroxisomes: an in vivo analysis uncovers a Ca(2+)-dependent scavenging system. Plant J. 62(5), 760-772. doi: 10.1111/j.1365-313X.2010.04190.x.

PubMed Central ID: 

Kim, T. H., Bohmer, M., Hu, H., Nishimura, N., Schroeder, J. I. (2010) Guard cell signal transduction network: advances in understanding Abscisic acid, CO2, and Ca2+ signaling. Annual Review of Plant Biology. 61, 561-91. doi: 10.1146/annurev-arplant-042809-112226.

PubMed Central ID: 

Yao, X., Horie, T., Xue, S., Leung, H. Y., Katsuhara, M., Brodsky, D. E., Wu, Y. & Schroeder, J. I. (2010) Differential sodium and potassium transport selectivities of the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells. Plant Physiol. 152, 341-55. doi: 10.1104/pp.109.145722.

PubMed Central ID: 

Nishimura, N., Sarkeshik, A., Nito, K., Park, S. Y., Wang, A., Carvalho, P. C., Lee, S., Caddell, D. F., Cutler, S. R., Chory, J., Yates, J. R., Schroeder, J. I. (2010) PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis. Plant J. 61, 290-299. doi: 10.1111/j.1365-313X.2009.04054.x.

PubMed Central ID: 

Horie, T., Hauser, F., Schroeder, J. I. (2009)  HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants. Trends Plant Sci. 14(12):660-8. doi: 10.1016/j.tplants.2009.08.009.

PubMed Central ID: 

Sung, D. Y., Kim, T. H., Komives, E. A., Mendoza-Cózatl, D. G., Schroeder, J. I. (2009)  ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis. Plant J. 59(5):802-13. doi: 10.1111/j.1365-313X.2009.03914.x.

PubMed Central ID: 

Nishimura, N., Hitomi, K., Arvai, A. S., Rambo, R. P., Hitomi, C., Cutler, S. R., Schroeder, J. I., Getzoff, E. D. (2009) Structural mechanism of abscisic acid binding and signaling by dimeric PYR1. Science. 326(5958), 1373-9. doi: 10.1126/science.1181829.

PubMed Central ID: 

Ward, J. M., Mäser, P., Schroeder, J. I. (2009)  Plant ion channels: gene families, physiology, and functional genomics analyses. Annu Rev Physio. 71, 59-82. doi: 10.1146/annurev.physiol.010908.163204.

PubMed Central ID: 

Kim, Y. Y., Kim, D. Y., Shim, D., Song, W. Y., Lee, J., Schroeder, J. I., Kim, S., Moran, N., Lee Y. (2008)  Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers Yeast. J Biol Chem. 283, 15893-15892. doi: 10.1074/jbc.M708947200.

PubMed Central ID: 

Vahisalu, T., Kollist, H., Wang, Y. F., Nishimura, N., Chan, W. Y., Valerio, G., Lamminmaki, A., Brosche, M., Moldau, H., Desikan, R., Schroeder, J. I., Kangasjarvi, J. (2008) SLAC1 is required for plant guard cell S‐type anion channel function in stomatal signalling. Nature. 452, 487‐91. doi: 10.1038/nature06608.

PubMed Central ID: 

Mendoza-Cozatl, D. G., Butko, E., Springer, F., Torpey, J. W., Komives, E. A., Kehr, J., Schroeder, J. I. (2008)  Identification of high levels of phytochelatins, glutathione and cadmium in the phloem sap of Brassica napus. A role for thiol-peptides in the long-distance transport of cadmium and the effect of cadmium on iron translocation. Plant Journal. 54,  249-59. 54(2):249-59. doi: 10.1111/j.1365-313X.2008.03410.x.

PubMed Central ID: 

Sadi, B. B., Vonderheide, A. P., Gong, J. M., Schroeder, J. I., Shann, J. R. & Caruso, J. A. (2008) An HPLC-ICP-MS technique for determination of cadmium-phytochelatins in genetically modified Arabidopsis thaliana. J Chromatogr B Analyt Technol Biomed Life Sci. 861,123-9. doi: 10.1016/j.jchromb.2007.11.004

PubMed Central ID: 

Imaizumi, T., Kay, S. A., Schroeder, J. I. (2007) Circadian rhythms. Daily watch on metabolism. Science. 318, 730-1731. doi: 10.1126/science.1151360

PubMed Central ID: 

Imaizumi, T., Schroeder, J. I., Kay, S. A. (2007) In SYNC: the ins and outs of circadian oscillations in calcium. Sci STKE. 390, pe32. doi: 10.1126/stke.3902007pe32.

PubMed Central ID: 

Horie, T., Costa, A., Kim, T. H., Han, M. H., Horie, R., Leung, H. Y., Miyao, A., Hirochika, H., An, G. and Schroeder, J.I. (2007) Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved roots for growth. EMBO J. 26, 3003-3014. doi: 10.1038/sj.emboj.7601732.

Sung, D. Y., Lee, D., Harris, H., Raab, A., Feldmann, J., Meharg, A., Kumabe, B., Komives, E. A., Schroeder, J. I. (2007)  Identification of an arsenic tolerant double mutant with a thiol-mediated component and increased arsenic tolerance in phyA mutants. Plant J49, 1064-107. doi: 10.1111/j.1365-313X.2006.03018.x.

Schroeder, J. I., Nambara, E. (2006) A quick release mechanism for abscisic acid. Cell. 126, 1023-1025. doi: 10.1016/j.cell.2006.09.001.

Platten, J. D., Cotsaftis, O., Berthomieu, P., Bohnert, H., Davenport, R. J., Fairbairn, D. J., Horie, T., Leigh, R.A., Lin, H. X., Luan, S., Maser, P., Pantoja, O., Rodriguez-Navarro, A., Schachtman, D. P., Schroeder, J. I., Sentenac, H., Uozumi, N., Very AA, Zhu JK, Dennis ES, Tester M. (2006) Nomenclature for HKT transporters, key determinants of plant salinity tolerance. Trends Plant Sci. 11, 372-374. doi: 10.1016/j.tplants.2006.06.001.

PubMed Central ID: 

Kwak, J. M., Nguyen, V., Schroeder, J. I. (2006) The role of reactive oxygen species in hormonal responses. Plant Physiology. 141, 323-329. doi: 10.1104/pp.106.079004

PubMed Central ID: 

Chen, A., Komives, E. A., Schroeder, J. I. (2006) An improved grafting technique for mature Arabidopsis plants demonstrates long-distance shoot-to-root transport of phytochelatins in Arabidopsis. Plant Physiology. 141, 108-120. doi: 10.1104/pp.105.072637.

Schroeder, J. I., Kuhn, J. M. (2006) Plant biology abscisic acid in bloom. Nature. 439, 277-278. doi: 10.1038/439277a

Sunarpi, Horie, T., Motoda, J., Kubo, M., Yang, H., Yoda, K., Horie, R., Chan, W., Leung, H. Y., Hattori, K., Konomi, M., Osumi, M., Yamagami, M., *Schroeder, J. I., *Uozumi, N. (* corresponding authors) (2005)  Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na+ unloading from xylem parenchyma cells. Pl Journal. 44, 928-938. doi: 10.1111/j.1365-313X.2005.02595.x

Li, Y., Dhankher, O. P., Carreira, L., Lee, D., Chen, A., Schroeder, J. I., Balish, R. S., Meagher, R. B. (2004) Overexpression of phytochelatin synthase in Arabidopsis leads to enhanced arsenic tolerance and cadmium hypersensitivity. Plant Cell Physiol. 45, 1787-1797. doi: 10.1093/pcp/pch202.

Wycisk, K., Kim, E. J., Schroeder, J. I., Kramer, U. (2004) Enhancing the First Enzymatic Step in Histidine Biosynthesis Pathway Increases the Free Histidine Pool and Nickel Tolerance in Arabidosis Thaliana. FEBS Letters. 578, 128-134. doi: 10.1016/j.febslet.2004.10.086

PubMed Central ID: 

Gong, J. M., Waner, D. A., Horie, T., Li, S. L., Horie, R., Abid, K. B., Schroeder, J. I. (2004) Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana. Proc Natl Acad Sci USA. 101, 15404-15409. doi:10.1073/pnas.0404780101

PubMed Central ID: 

Mori, I. and Schroeder, J. I. (2004)  Reactive Oxygen Species Activation of Plant Ca2+ Channels: A Signaling Mechanism in Polar Growth, Hormone Transduction, Stress Signaling, and Hypothetically Mechano-transduction. Plant Physiology 135(2), 702-708. doi: 10.1104/pp.104.042069.

Lee, D. A., Chen, A., Schroeder, J. I. (2003) ars1, an Arabidopsis mutant exhibiting increased tolerance to arsenate and increased phosphate uptake. Plant J. 35(5), 637-46.

PubMed Central ID: 

Gong, J. M., Lee, D. A., Schroeder, J. I. (2003) Long-distance root-to-shoot transport of phytochelatins and cadmium in Arabidopsis. Proc Natl Acad Sci USA. 100(17), 10118-23. doi: 10.1073/pnas.1734072100.

Mäser, P., Eckelman, B., Vaidyanathan, R., Horie, T., Fairbairn, D. J., Kubo, M., Yamagami, M., Yamaguchi, K., Nishimura, M., Uozumi, N., Robertson, W., Sussman, M. R., Schroeder, J. I. (2002) Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1. FEBS Lett. 531(2), 157-61.

PubMed Central ID: 

Allen, G. J., Murata, Y., Chu, S. P., Nafisi, M., Schroeder, J. I. (2002) Hypersensitivity of abscisic acid-induced cytosolic calcium increases in the Arabidopsis farnesyltransferase mutant era1-2. Plant Cell. 14(7), 1649-62. doi: 10.1105/tpc.010448.

PubMed Central ID: 

Mäser, P., Hosoo, Y., Goshima, S., Horie, T., Eckelman, B., Yamada, K., Yoshida, K., Bakker, E. P., Shinmyo, A., Oiki, S., Schroeder, J. I., Uozumi, N. (2002) Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants. Proc Natl Acad Sci USA. 99(9), 6428-33. doi: 10.1073/pnas.082123799.

Ghassemian, M., Waner, D., Tchieu, J., Gribskov, M., Schroeder, J. I. (2001) An integrated Arabidopsis annotation database for Affymetrix Genechip data analysis, and tools for regulatory motif searches. Trends Plant Sci. 6(10), 448-9. doi: 10.1016/S1360-1385(01)02092-1.

Clemens, S., Schroeder, J. I., Degenkolb, T. (2001) Caenorhabditis elegans expresses a functional phytochelatin synthase. Eur J Biochem. 268(13), 3640-3. doi: 10.1046/j.1432-1327.2001.02293.x.

 

Main Contact Information

Dr. Julian I. Schroeder (Torrey Mesa Research Institute Chair in Plant Science, Department of Biology and Bioengineering-UCSD) 

Contact

UCSD Superfund Research Center
University of California, San Diego
Pharmacology Department
9500 Gilman Drive, Mail Code 0722
La Jolla, CA 92093-0722